A parallel microfluidic flow cytometer for high-content screening.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 21478861.
- Also identified by DOI 10.1038/nmeth.1595 and PMC identifier 3084896.
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Abstract
A parallel microfluidic cytometer (PMC) uses a high-speed scanning photomultiplier-based detector to combine low-pixel-count, one-dimensional imaging with flow cytometry. The 384 parallel flow channels of the PMC decouple count rate from signal-to-noise ratio. Using six-pixel one-dimensional images, we investigated protein localization in a yeast model for human protein misfolding diseases and demonstrated the feasibility of a nuclear-translocation assay in Chinese hamster ovary (CHO) cells expressing an NFκB-EGFP reporter.
Medical subject headings
- Flow Cytometry
- Microfluidic Analytical Techniques