Bismark: a flexible aligner and methylation caller for Bisulfite-Seq applications.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 21493656.
- Also identified by DOI 10.1093/bioinformatics/btr167 and PMC identifier 3102221.
- Licence recorded as CC BY-NC.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
A combination of bisulfite treatment of DNA and high-throughput sequencing (BS-Seq) can capture a snapshot of a cell's epigenomic state by revealing its genome-wide cytosine methylation at single base resolution. Bismark is a flexible tool for the time-efficient analysis of BS-Seq data which performs both read mapping and methylation calling in a single convenient step. Its output discriminates between cytosines in CpG, CHG and CHH context and enables bench scientists to visualize and interpret their methylation data soon after the sequencing run is completed. Bismark is released under the GNU GPLv3+ licence. The source code is freely available from www.bioinformatics.bbsrc.ac.uk/projects/bismark/.
Medical subject headings
- DNA Methylation
- Sequence Analysis, DNA
- Software
- Sulfites