Protein-protein fusion catalyzed by sortase A.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 21494692.
- Also identified by DOI 10.1371/journal.pone.0018342 and PMC identifier 3071835.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Chimeric proteins boast widespread use in areas ranging from cell biology to drug delivery. Post-translational protein fusion using the bacterial transpeptidase sortase A provides an attractive alternative when traditional gene fusion fails. We describe use of this enzyme for in vitro protein ligation and report the successful fusion of 10 pairs of protein domains with preserved functionality--demonstrating the robust and facile nature of this reaction.
Medical subject headings
- Aminoacyltransferases
- Bacterial Proteins
- Biocatalysis
- Cysteine Endopeptidases
- Recombinant Fusion Proteins