Next-generation sequencing to generate interactome datasets.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 21516116.
- Also identified by DOI 10.1038/nmeth.1597 and PMC identifier 3188388.
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Abstract
Next-generation sequencing has not been applied to protein-protein interactome network mapping so far because the association between the members of each interacting pair would not be maintained in en masse sequencing. We describe a massively parallel interactome-mapping pipeline, Stitch-seq, that combines PCR stitching with next-generation sequencing and used it to generate a new human interactome dataset. Stitch-seq is applicable to various interaction assays and should help expand interactome network mapping.
Medical subject headings
- Databases, Protein
- Protein Interaction Mapping
- Sequence Analysis, DNA