Combining quantitative proteomics data processing workflows for greater sensitivity.

Colaert, Niklaas; Van Huele, Christophe; Degroeve, Sven; Staes, An; Vandekerckhove, Joël; Gevaert, Kris; Martens, Lennart · Nat Methods · 2011

basic_science · Level V

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Abstract

We here describe a normalization method to combine quantitative proteomics data. By merging the output of two popular quantification software packages, we obtained a 20% increase (on average) in the number of quantified human proteins without suffering from a loss of quality. Our integrative workflow is freely available through our user-friendly, open-source Rover software (http://compomics-rover.googlecode.com/).

Medical subject headings