Method to identify genomic targets of DNA binding proteins.
basic_science · Level V
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- Record sourced from PubMed, PMID 2159142.
- Also identified by PMC identifier 53882.
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Abstract
We have devised a cyclical immunoprecipitation protocol that can be used to identify and clone a specific DNA sequence that is recognized by a DNA binding protein, even if that sequence is present in only one copy in the genome of a mammal. As an example, we have used this procedure to purify mouse genomic sequences to which the simian virus 40 tumor (T) antigen binds.
Medical subject headings
- Antigens, Polyomavirus Transforming
- DNA
- DNA-Binding Proteins
- Genomic Library
- Plasmids