Rapid screening of complex DNA samples by single-molecule amplification and sequencing.
basic_science · Level V
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- Record sourced from PubMed, PMID 21625543.
- Also identified by DOI 10.1371/journal.pone.0019723 and PMC identifier PMC2910068.
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Abstract
Microbial cloning makes Sanger sequencing of complex DNA samples possible but is labor intensive. We present a simple, rapid and robust method that enables laboratories without special equipment to perform single-molecule amplicon sequencing, although in a low-throughput manner, from sub-picogram quantities of DNA. The method can also be used for quick quality control of next-generation sequencing libraries, as was demonstrated for a metagenomic sample.