Sharper low-power STED nanoscopy by time gating.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 21642963.
- Also identified by DOI 10.1038/nmeth.1624.
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Abstract
Applying pulsed excitation together with time-gated detection improves the fluorescence on-off contrast in continuous-wave stimulated emission depletion (CW-STED) microscopy, thus revealing finer details in fixed and living cells using moderate light intensities. This method also enables super-resolution fluorescence correlation spectroscopy with CW-STED beams, as demonstrated by quantifying the dynamics of labeled lipid molecules in the plasma membrane of living cells.
Medical subject headings
- Microscopy, Fluorescence