Single-tube linear DNA amplification (LinDA) for robust ChIP-seq.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 21642965.
- Also identified by DOI 10.1038/nmeth.1626.
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Abstract
Genome-wide profiling of transcription factors based on massive parallel sequencing of immunoprecipitated chromatin (ChIP-seq) requires nanogram amounts of DNA. Here we describe a high-fidelity, single-tube linear DNA amplification method (LinDA) for ChIP-seq and reChIP-seq with picogram DNA amounts obtained from a few thousand cells. This amplification technology will facilitate global analyses of transcription-factor binding and chromatin with very small cell populations, such as stem or cancer-initiating cells.
Medical subject headings
- Chromatin
- Chromatin Immunoprecipitation
- DNA
- Nucleic Acid Amplification Techniques
- Sequence Analysis, DNA
- Transcription Factors