Near-infrared branding efficiently correlates light and electron microscopy.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 21642966.
- Also identified by DOI 10.1038/nmeth.1622.
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Abstract
The correlation of light and electron microscopy of complex tissues remains a major challenge. Here we report near-infrared branding (NIRB), which facilitates such correlation by using a pulsed, near-infrared laser to create defined fiducial marks in three dimensions in fixed tissue. As these marks are fluorescent and can be photo-oxidized to generate electron contrast, they can guide re-identification of previously imaged structures as small as dendritic spines by electron microscopy.
Medical subject headings
- Fiducial Markers
- Infrared Rays
- Lasers
- Microscopy
- Microscopy, Electron