Pathogen detection using short-RNA deep sequencing subtraction and assembly.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 21666269.
- Also identified by DOI 10.1093/bioinformatics/btr349 and PMC identifier 3137223.
- Licence recorded as CC BY-NC.
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Abstract
Early and accurate detection of human pathogen infection is critical for treatment and therapeutics. Here we describe pathogen identification using short RNA subtraction and assembly (SRSA), a detection method that overcomes the requirement of prior knowledge and culturing of pathogens, by using degraded small RNA and deep sequencing technology. We prove our approach's efficiency through identification of a combined viral and bacterial infection in human cells.
Medical subject headings
- High-Throughput Nucleotide Sequencing
- Sequence Analysis, RNA