Ambidextrous magnetic nanovectors for synchronous gene transfection and labeling of human MSCs.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 21696819.
- Also identified by DOI 10.1016/j.biomaterials.2011.04.007.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
The synchronization of gene expression and cell trafficking in transfected stem cells is crucial for augmentation of stem cell functions (differentiation and neurotropic factor secretion) and real time in vivo monitoring. We report a magnetic nanoparticle-based gene delivery system that can ensure simultaneous gene delivery and in vivo cell trafficking by high resolution MR imaging. The polar aprotic solvent soluble MnFe₂O₄ nanoparticles were enveloped using cationic polymers (branched polyethyleneimine, PEI) by the solvent shifting method for a gene loading. Using our magnetic nanovector system (PEI-coated MnFe₂O₄ nanoparticles), thus, we synchronized stem cell migration and its gene expression in a rat stroke model.
Medical subject headings
- Genetic Vectors
- Magnetics
- Mesenchymal Stem Cells
- Nanoparticles
- Transfection