The avian retroviral IN protein is both necessary and sufficient for integrative recombination in vitro.
basic_science · Level V
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Abstract
The integration of viral DNA into the host cell chromosome is an essential feature of the retroviral life cycle. The integration reaction requires cis-acting sequences at the ends of linear viral DNA and a trans-acting product of the pol gene, the integration protein (IN). Previously, we demonstrated that avian sarcoma-leukosis virus (ASLV) IN is able to carry out the first step in the integration process in vitro: nicking of the ends of linear viral DNA. In this paper, using two independent assays, we demonstrate that IN, alone, is sufficient to carry out the second step: cleavage and joining to the target DNA. These results demonstrate that the retroviral IN protein is an integrase.
Medical subject headings
- Alpharetrovirus
- DNA Nucleotidyltransferases
- DNA, Viral
- Recombination, Genetic