A public genome-scale lentiviral expression library of human ORFs.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 21706014.
- Also identified by DOI 10.1038/nmeth.1638 and PMC identifier 3234135.
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Abstract
Functional characterization of the human genome requires tools for systematically modulating gene expression in both loss-of-function and gain-of-function experiments. We describe the production of a sequence-confirmed, clonal collection of over 16,100 human open-reading frames (ORFs) encoded in a versatile Gateway vector system. Using this ORFeome resource, we created a genome-scale expression collection in a lentiviral vector, thereby enabling both targeted experiments and high-throughput screens in diverse cell types.
Medical subject headings
- Cloning, Molecular
- Genetic Vectors
- Genomic Library
- Lentivirus