Biocompatible artificial DNA linker that is read through by DNA polymerases and is functional in Escherichia coli.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 21709264.
- Also identified by DOI 10.1073/pnas.1101519108 and PMC identifier 3136318.
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Abstract
A triazole mimic of a DNA phosphodiester linkage has been produced by templated chemical ligation of oligonucleotides functionalized with 5'-azide and 3'-alkyne. The individual azide and alkyne oligonucleotides were synthesized by standard phosphoramidite methods and assembled using a straightforward ligation procedure. This highly efficient chemical equivalent of enzymatic DNA ligation has been used to assemble a 300-mer from three 100-mer oligonucleotides, demonstrating the total chemical synthesis of very long oligonucleotides. The base sequences of the DNA strands containing this artificial linkage were copied during PCR with high fidelity and a gene containing the triazole linker was functional in Escherichia coli.
Medical subject headings
- DNA