Gene delivery to tumor cells by cationic polymeric nanovectors coupled to folic acid and the cell-penetrating peptide octaarginine.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 21715001.
- Also identified by DOI 10.1016/j.biomaterials.2011.06.015.
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Abstract
Target ligand folic acid (FA) and cell-penetrating peptide octaarginine (R8) were coupled with the gene vectors (PEI(600)-CyD, PC) composed of β-cyclodextrin (β-CyD) and low-molecular-weight polyethylenimine (PEI, Mw 600) to form nanovectors for highly efficient gene delivery to tumor cells. The resultant ternary nanocomplexes of FA-PC/R8-PC/pDNA produced excellent gene transfaction abilities in the folate receptor (FR)-positive tumor cells in vitro and in vivo. The FR-mediated endocytosis and the R8-mediated transmembrane functionality together contributed to the high transfection levels. This study provides a promising means to produce gene nanovectors for in vivo applications.
Medical subject headings
- Cations
- Folic Acid
- Gene Transfer Techniques
- Genetic Vectors
- Nanostructures
- Oligopeptides
- Polymers