Megapixel digital PCR.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 21725299.
- Also identified by DOI 10.1038/nmeth.1640.
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Abstract
We present a microfluidic 'megapixel' digital PCR device that uses surface tension-based sample partitioning and dehydration control to enable high-fidelity single DNA molecule amplification in 1,000,000 reactors of picoliter volume with densities up to 440,000 reactors cm(-2). This device achieves a dynamic range of 10(7), single-nucleotide-variant detection below one copy per 100,000 wild-type sequences and the discrimination of a 1% difference in chromosome copy number.
Medical subject headings
- DNA Mutational Analysis
- Gene Expression Profiling
- Microfluidics
- Polymerase Chain Reaction