Two-photon polarization microscopy reveals protein structure and function.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 21725301.
- Also identified by DOI 10.1038/nmeth.1643.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
Membrane proteins are a large, diverse group of proteins, serving a multitude of cellular functions. They are difficult to study because of their requirement of a lipid membrane for function. Here we show that two-photon polarization microscopy can take advantage of the cell membrane requirement to yield insights into membrane protein structure and function, in living cells and organisms. The technique allows sensitive imaging of G-protein activation, changes in intracellular calcium concentration and other processes, and is not limited to membrane proteins. Conveniently, many suitable probes for two-photon polarization microscopy already exist.
Medical subject headings
- Cell Membrane
- Membrane Proteins
- Microscopy, Fluorescence, Multiphoton
- Microscopy, Polarization