Deep and fast live imaging with two-photon scanned light-sheet microscopy.
basic_science · Level V
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- Record sourced from PubMed, PMID 21765409.
- Also identified by DOI 10.1038/nmeth.1652.
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Abstract
We implemented two-photon scanned light-sheet microscopy, combining nonlinear excitation with orthogonal illumination of light-sheet microscopy, and showed its excellent performance for in vivo, cellular-resolution, three-dimensional imaging of large biological samples. Live imaging of fruit fly and zebrafish embryos confirmed that the technique can be used to image up to twice deeper than with one-photon light-sheet microscopy and more than ten times faster than with point-scanning two-photon microscopy without compromising normal biology.
Medical subject headings
- Embryo, Nonmammalian
- Imaging, Three-Dimensional
- Microscopy