Dual-label STED nanoscopy of living cells using photochromism.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 21786833.
- Also identified by DOI 10.1021/nl202290w.
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Abstract
We demonstrate live-cell STED microscopy of two protein species using photochromic green fluorescent proteins as markers. The reversible photoswitching of two markers is implemented so that they can be discerned with a single excitation and STED wavelength and a single detection channel. Dual-label STED microscopy is shown in living mammalian cells.
Medical subject headings
- Microscopy, Fluorescence
- Nanotechnology
- Photochemistry