Male germ cells express abundant endogenous siRNAs.
basic_science · Level V
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- Record sourced from PubMed, PMID 21788498.
- Also identified by DOI 10.1073/pnas.1108567108 and PMC identifier 3156200.
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Abstract
In mammals, endogenous siRNAs (endo-siRNAs) have only been reported in murine oocytes and embryonic stem cells. Here, we show that murine spermatogenic cells express numerous endo-siRNAs, which are likely to be derived from naturally occurring double-stranded RNA (dsRNA) precursors. The biogenesis of these testicular endo-siRNAs is DROSHA independent, but DICER dependent. These male germ cell endo-siRNAs can potentially target hundreds of transcripts or thousands of DNA regions in the genome. Overall, our work has unveiled another hidden layer of regulation imposed by small noncoding RNAs during male germ cell development.
Medical subject headings
- RNA, Small Interfering
- Spermatozoa