Quantitative photo activated localization microscopy: unraveling the effects of photoblinking.
basic_science · Level V
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- Record sourced from PubMed, PMID 21818365.
- Also identified by DOI 10.1371/journal.pone.0022678 and PMC identifier PMC1157093.
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Abstract
In this work we discuss how to use photophysical information for improved quantitative measurements using Photo Activated Localization Microscopy (PALM) imaging. We introduce a method that reliably estimates the number of photoblinking molecules present in a biological sample and gives a robust way to quantify proteins at the single-cell level from PALM images. We apply this method to determine the amount of β2 adrenergic receptor, a prototypical G Protein Coupled Receptor, expressed on the plasma membrane of HeLa cells.