Checkpoints in adenoviral production: cross-contamination and E1A.
basic_science · Level V
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- Record sourced from PubMed, PMID 21826235.
- Also identified by DOI 10.1371/journal.pone.0023160 and PMC identifier PMC2817600.
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Abstract
Adenoviruses are widely used for overexpressing proteins in primary mammalian cells. Incorporation of the early viral gene, E1A, or viral cross-contamination can occur during amplification, and identification of these products is crucial as the transcription of unwanted genetic material can impact cell function and compromise data interpretation. Here we report methods for evaluation of contaminating adenovirus and E1 viral DNA.