Transcriptomic analysis brings new insight into the biological role of the prion protein during mouse embryogenesis.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 21858045.
- Also identified by DOI 10.1371/journal.pone.0023253 and PMC identifier 3156130.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
The biological function of the Prion protein remains largely unknown but recent data revealed its implication in early zebrafish and mammalian embryogenesis. To gain further insight into its biological function, comparative transcriptomic analysis between FVB/N and FVB/N Prnp knockout mice was performed at early embryonic stages. RNAseq analysis revealed the differential expression of 73 and 263 genes at E6.5 and E7.5, respectively. The related metabolic pathways identified in this analysis partially overlap with those described in PrP1 and PrP2 knockdown zebrafish embryos and prion-infected mammalian brains and emphasize a potentially important role for the PrP family genes in early developmental processes.
Medical subject headings
- Animals
- Embryo, Mammalian
- Embryo, Mammalian/embryology
- Embryo, Mammalian/metabolism
- Embryo, Nonmammalian
- Embryo, Nonmammalian/embryology
- Embryo, Nonmammalian/metabolism
- Embryonic Development
- Embryonic Development/genetics
- Gene Expression Profiling
- Gene Expression Profiling/methods
- Gene Expression Regulation, Developmental
- Gene Knockdown Techniques
- Gene Regulatory Networks
- Immunohistochemistry
- Mice
- Mice, Knockout
- Prions
- Prions/genetics
- Prions/metabolism
- Protein Isoforms
- Protein Isoforms/genetics
- Protein Isoforms/metabolism
- Reverse Transcriptase Polymerase Chain Reaction
- Transcriptome
- Zebrafish
- Zebrafish/embryology
- Zebrafish/genetics
- Zebrafish/metabolism
- Zebrafish Proteins
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism