Nuclear pore complex protein mediated nuclear localization of dicer protein in human cells.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 21858095.
- Also identified by DOI 10.1371/journal.pone.0023385 and PMC identifier 3156128.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Human DICER1 protein cleaves double-stranded RNA into small sizes, a crucial step in production of single-stranded RNAs which are mediating factors of cytoplasmic RNA interference. Here, we clearly demonstrate that human DICER1 protein localizes not only to the cytoplasm but also to the nucleoplasm. We also find that human DICER1 protein associates with the NUP153 protein, one component of the nuclear pore complex. This association is detected predominantly in the cytoplasm but is also clearly distinguishable at the nuclear periphery. Additional characterization of the NUP153-DICER1 association suggests NUP153 plays a crucial role in the nuclear localization of the DICER1 protein.
Medical subject headings
- Cell Nucleus
- DEAD-box RNA Helicases
- Nuclear Pore Complex Proteins
- Ribonuclease III