Quantitative proteomics by amino acid labeling in C. elegans.
basic_science · Level V
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- Record sourced from PubMed, PMID 21874006.
- Also identified by DOI 10.1038/nmeth.1675.
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Abstract
We demonstrate labeling of Caenorhabditis elegans with heavy isotope-labeled lysine by feeding them with heavy isotope-labeled Escherichia coli. Using heavy isotope-labeled worms and quantitative proteomics methods, we identified several proteins that are regulated in response to loss or RNAi-mediated knockdown of the nuclear hormone receptor 49 in C. elegans. The combined use of quantitative proteomics and selective gene knockdown is a powerful tool for C. elegans biology.
Medical subject headings
- Caenorhabditis elegans
- Caenorhabditis elegans Proteins
- Lysine
- Proteome
- Proteomics