Stable-isotope labeling with amino acids in nematodes.
basic_science · Level V
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- Record sourced from PubMed, PMID 21874007.
- Also identified by DOI 10.1038/nmeth.1679 and PMC identifier 3184259.
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Abstract
We describe an approach for accurate quantitation of global protein dynamics in Caenorhabditis elegans. We adapted stable-isotope labeling with amino acids in cell culture (SILAC) for nematodes by feeding worms a heavy lysine- and heavy arginine-labeled Escherichia coli strain and report a genetic solution to elminate the problem of arginine-to-proline conversion. Combining our approach with quantitative proteomics methods, we characterized the heat-shock response in worms.
Medical subject headings
- Arginine
- Caenorhabditis elegans
- Isotope Labeling
- Lysine