Three-dimensional biomaterials for the study of human pluripotent stem cells.

Kraehenbuehl, Thomas P; Langer, Robert; Ferreira, Lino S · Nat Methods · 2011

basic_science · Level V

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Abstract

The self-renewal and differentiation of human pluripotent stem cells (hPSCs) have typically been studied in flat, two-dimensional (2D) environments. In this Perspective, we argue that 3D model systems may be needed in addition, as they mimic the natural 3D tissue organization more closely. We survey methods that have used 3D biomaterials for expansion of undifferentiated hPSCs, directed differentiation of hPSCs and transplantation of differentiated hPSCs in vivo.

Medical subject headings