Two-photon 3D FIONA of individual quantum dots in an aqueous environment.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 21882883.
- Also identified by DOI 10.1021/nl201225r and PMC identifier 3192306.
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Abstract
We report the first two-photon (2P) microscopy of individual quantum dots (QDs) in an aqueous environment with both widefield and point-scan excitations at nanometer accuracy. Thiol-containing reductants suppress QD blinking and enable measurement of the 36 nm step size of individual Myosin V motors in vitro. We localize QDs with an accuracy of 2-3 nm in all three dimensions by using a 9 × 9 matrix excitation hologram and an array detector, which also increases the 3D scan imaging rate by 80-fold. With this 3D microscopy we validate the LamB receptor distribution on E. coli and the endocytosis of EGF-receptors in breast cancer cells.
Medical subject headings
- Quantum Dots
- Water