Mediator subunit 12 is required for neutrophil development in zebrafish.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 21901140.
- Also identified by DOI 10.1371/journal.pone.0023845 and PMC identifier 3162013.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Hematopoiesis requires the spatiotemporal organization of regulatory factors to successfully orchestrate diverse lineage specificity from stem and progenitor cells. Med12 is a regulatory component of the large Mediator complex that enables contact between the general RNA polymerase II transcriptional machinery and enhancer bound regulatory factors. We have identified a new zebrafish med12 allele, syr, with a single missense mutation causing a valine to aspartic acid change at position 1046. Syr shows defects in hematopoiesis, which predominantly affect the myeloid lineage. Syr has identified a hematopoietic cell-specific requirement for Med12, suggesting a new role for this transcriptional regulator.
Medical subject headings
- Mediator Complex
- Neutrophils
- Zebrafish
- Zebrafish Proteins