Light-based feedback for controlling intracellular signaling dynamics.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 21909100.
- Also identified by DOI 10.1038/nmeth.1700 and PMC identifier 3184382.
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Abstract
The ability to apply precise inputs to signaling species in live cells would be transformative for interrogating and understanding complex cell-signaling systems. Here we report an 'optogenetic' method for applying custom signaling inputs using feedback control of a light-gated protein-protein interaction. We applied this strategy to perturb protein localization and phosphoinositide 3-kinase activity, generating time-varying signals and clamping signals to buffer against cell-to-cell variability or changes in pathway activity.
Medical subject headings
- Feedback, Physiological
- Light
- Signal Transduction