High-efficiency screening of monoclonal antibodies for membrane protein crystallography.
basic_science · Level V
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- Record sourced from PubMed, PMID 21931797.
- Also identified by DOI 10.1371/journal.pone.0024653 and PMC identifier PMC508830.
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Abstract
Determination of crystal structures of membrane proteins is often limited by difficulties obtaining crystals diffracting to high resolution. Co-crystallization with Fab fragments of monoclonal antibodies has been reported to improve diffraction of membrane proteins crystals. However, it is not simple to generate useful monoclonal antibodies for membrane protein crystallography. In this report, we present an optimized process for efficient screening from immunization to final validation of monoclonal antibody for membrane protein crystallography.