Measuring protein structure and stability of protein-nanoparticle systems with synchrotron radiation circular dichroism.
basic_science · Level V
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- Record sourced from PubMed, PMID 21932791.
- Also identified by DOI 10.1021/nl202909s.
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Abstract
We measure the structural and stability changes of proteins at nanomolar concentration upon interaction with nanoparticles. Using synchrotron radiation circular dichroism (SRCD), we measure a decrease of 6 °C in the thermal unfolding of human serum albumin upon interaction with silver nanoparticles while this does not happen with gold. The use of SRCD allows measuring critical parameters on protein-nanoparticle interactions, and it will provide experimental data on the relative stability of key biological proteins for nanotoxicology.
Medical subject headings
- Circular Dichroism
- Metal Nanoparticles
- Proteins
- Synchrotrons