Isolation of DNA aptamers using micro free flow electrophoresis.
basic_science · Level V
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- Record sourced from PubMed, PMID 21947169.
- Also identified by DOI 10.1039/c1lc20461k and PMC identifier 3454500.
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Abstract
A micro free flow electrophoresis (μFFE) device was used to select DNA aptamers for human immunoglobulin E (IgE). The continuous nature of μFFE allowed 1.8 × 10(14) sequences to be introduced over a period of 30 min, a 300-fold improvement in library size over capillary electrophoresis based selections (CE-SELEX). Four rounds of selection were performed within four days. Aptamers with low nM dissociation constants for IgE were identified after a single round of μFFE selection.
Medical subject headings
- Aptamers, Nucleotide
- Electrophoresis, Capillary
- SELEX Aptamer Technique