MS3 eliminates ratio distortion in isobaric multiplexed quantitative proteomics.
basic_science · Level V
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- Record sourced from PubMed, PMID 21963607.
- Also identified by DOI 10.1038/nmeth.1714 and PMC identifier 3205343.
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Abstract
Quantitative mass spectrometry-based proteomics is highly versatile but not easily multiplexed. Isobaric labeling strategies allow mass spectrometry-based multiplexed proteome quantification; however, ratio distortion owing to protein quantification interference is a common effect. We present a two-proteome model (mixture of human and yeast proteins) in a sixplex isobaric labeling system to fully document the interference effect, and we report that applying triple-stage mass spectrometry (MS3) almost completely eliminates interference.
Medical subject headings
- Proteomics