Lateral dielectrophoretic microseparators to measure the size distribution of blood cells.

Abstract

Lateral displacement of blood cells occurred when they were passed over a planar interdigitated electrode array placed at an angle to the direction of flow, and was determined to be a function of cell size. A simplified line charge model was used to estimate numerically the lateral displacement. Based on the size-specific lateral displacement, a lateral dielectrophoretic (DEP) microseparator was developed to measure the size distribution of blood cells using fluorescence microscopy. To determine whether the lateral DEP microseparator was useful, it was used to detect acute leukemia by measuring the size distribution of blood cells. The lateral DEP microseparator provided a practical method for continuously and simultaneously separating multi-cell populations by size from a heterogeneous cell population. In the future, sensitivity of the lateral DEP microseparator could be improved and it could be automated by integrating subsequent advanced detection technologies in a micro-format.