Reactive oxygen species driven angiogenesis by inorganic nanorods.

Patra, Chitta Ranjan; Kim, Jong-Ho; Pramanik, Kallal; d'Uscio, Livius V; Patra, Sujata; Pal, Krishnendu; Ramchandran, Ramani; Strano, Michael S et al. · Nano Lett · 2011

basic_science · Level V

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Abstract

The exact mechanism of angiogenesis by europium hydroxide nanorods was unclear. In this study we have showed that formation of reactive oxygen species (H(2)O(2) and O(2)·-) is involved in redox signaling pathways during angiogenesis, important for cardiovascular and ischemic diseases. Here we used single-walled carbon nanotube sensor array to measure the single-molecule efflux of H(2)O(2) and a HPLC method for the determination of O(2)·- from endothelial cells in response to proangiogenic factors. Additionally, reactive oxygen species-mediated angiogenesis using inorganic nanorods was observed in transgenic (fli1a:EGFP) zebrafish embryos.

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