Live-cell 3D super-resolution imaging in thick biological samples.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 21983925.
- Also identified by DOI 10.1038/nmeth.1744.
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Abstract
We demonstrate three-dimensional (3D) super-resolution live-cell imaging through thick specimens (50-150 μm), by coupling far-field individual molecule localization with selective plane illumination microscopy (SPIM). The improved signal-to-noise ratio of selective plane illumination allows nanometric localization of single molecules in thick scattering specimens without activating or exciting molecules outside the focal plane. We report 3D super-resolution imaging of cellular spheroids.
Medical subject headings
- Imaging, Three-Dimensional
- Microscopy, Fluorescence
- Spheroids, Cellular