Knockdown of Ebola virus VP24 impairs viral nucleocapsid assembly and prevents virus replication.

Mateo, Mathieu; Carbonnelle, Caroline; Martinez, Miguel J; Reynard, Olivier; Page, Audrey; Volchkova, Valentina A; Volchkov, Viktor E · J Infect Dis · 2011

basic_science · Level V

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Abstract

The structural protein VP24 of Ebola virus (EBOV) is a determinant of virulence in rodent models and possesses an interferon antagonist function. In this study, we investigate the role of VP24 in EBOV replication using RNA interference by small interfering RNA to knock down the expression of this protein in virus-infected cells. We reveal that VP24 is required for assembly of viral nucleocapsids and that silencing of VP24 expression prevents the release of EBOV.

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