Label-free real-time imaging in microchip free-flow electrophoresis applying high speed deep UV fluorescence scanning.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 22011722.
- Also identified by DOI 10.1039/c1lc20558g.
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Abstract
We report on label-free monitoring of microfluidic free-flow electrophoresis (μFFE) separations in real-time using a custom built high speed deep UV laser scanner. In combination with a novel layout realized in fused silica (FS) FFE chips the setup was successfully applied for continuous separations and detection of unlabeled analytes including native proteins by space-resolved intrinsic deep UV fluorescence scanning.
Medical subject headings
- Electrophoresis, Microchip
- Spectrometry, Fluorescence