NKX2-5(eGFP/w) hESCs for isolation of human cardiac progenitors and cardiomyocytes.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 22020065.
- Also identified by DOI 10.1038/nmeth.1740.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
NKX2-5 is expressed in the heart throughout life. We targeted eGFP sequences to the NKX2-5 locus of human embryonic stem cells (hESCs); NKX2-5(eGFP/w) hESCs facilitate quantification of cardiac differentiation, purification of hESC-derived committed cardiac progenitor cells (hESC-CPCs) and cardiomyocytes (hESC-CMs) and the standardization of differentiation protocols. We used NKX2-5 eGFP(+) cells to identify VCAM1 and SIRPA as cell-surface markers expressed in cardiac lineages.
Medical subject headings
- Cell Separation
- Embryonic Stem Cells
- Green Fluorescent Proteins
- Homeodomain Proteins
- Myoblasts, Cardiac
- Myocytes, Cardiac
- Transcription Factors