Broadband cavity-enhanced absorption spectroscopy for real time, in situ spectral analysis of microfluidic droplets.

Neil, Simon R T; Rushworth, Cathy M; Vallance, Claire; Mackenzie, Stuart R · Lab Chip · 2011

basic_science · Level V

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Abstract

Broadband cavity-enhanced absorption spectroscopy has been used to record, in real time, the absorption spectrum of microlitre volume aqueous phase droplets within a microfluidic chip assembly. Using supercontinuum radiation and broadband coated external mirrors, the full visible spectrum (430 nm < λ < 700 nm) of each passing droplet is acquired in situ at high repetition rates (273 Hz/3.66 ms acquisition time) and high sensitivity (α(min) < 10(-2) cm(-1)). The possibilities for further improvements in sensitivity and acquisition rate using custom designed chips are discussed.