A cell population structuring model to estimate recombinant strain growth in a closed system for subsequent search of the mode to increase protein accumulation during protealysin producer cultivation.
basic_science · Level V
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- Record sourced from PubMed, PMID 22027278.
- Also identified by DOI 10.1088/1758-5082/3/4/045006.
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Abstract
In this paper we have proposed a new structured population growth model, further developing a model previously proposed by the authors. Based on this model, optimal growth characteristics of the recombinant strain Escherichia coli BL-21 (DE3) [pProPlnHis(6)] were determined, which allowed us to increase the output of metalloproteinase by 300%. We have experimentally demonstrated the applicability of the new model to cell cultures with implanted plasmids and the potential practical use for an output increase of a wide variety of biosynthesis processes.
Medical subject headings
- Cell Culture Techniques
- Escherichia coli
- Escherichia coli Proteins
- Genetic Engineering
- Metalloproteases
- Models, Biological