Yeast one-hybrid assays for gene-centered human gene regulatory network mapping.
basic_science · Level V
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- Record sourced from PubMed, PMID 22037702.
- Also identified by DOI 10.1038/nmeth.1764 and PMC identifier 3263363.
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Abstract
Gateway-compatible yeast one-hybrid (Y1H) assays provide a convenient gene-centered (DNA to protein) approach to identify transcription factors that can bind a DNA sequence of interest. We present Y1H resources, including clones for 988 of 1,434 (69%) predicted human transcription factors, that can be used to detect both known and new interactions between human DNA regions and transcription factors.
Medical subject headings
- Gene Regulatory Networks
- Genes
- Two-Hybrid System Techniques