Enhanced Y1H assays for Arabidopsis.
basic_science · Level V
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- Record sourced from PubMed, PMID 22037706.
- Also identified by DOI 10.1038/nmeth.1750 and PMC identifier 3821074.
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Abstract
We present an Arabidopsis thaliana full-length transcription factor resource of 92% of root stele-expressed transcription factors and 74.5% of root-expressed transcription factors. We demonstrate its use with enhanced yeast one-hybrid (eY1H) screening for rapid, systematic mapping of plant transcription factor-promoter interactions. We identified 158 interactions with 13 stele-expressed promoters, many of which occur physically or are regulatory in planta.
Medical subject headings
- Arabidopsis
- Promoter Regions, Genetic
- Transcription Factors
- Two-Hybrid System Techniques