In silico feedback for in vivo regulation of a gene expression circuit.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 22057053.
- Also identified by DOI 10.1038/nbt.2018 and PMC identifier 4565053.
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Abstract
We show that difficulties in regulating cellular behavior with synthetic biological circuits may be circumvented using in silico feedback control. By tracking a circuit's output in Saccharomyces cerevisiae in real time, we precisely control its behavior using an in silico feedback algorithm to compute regulatory inputs implemented through a genetically encoded light-responsive module. Moving control functions outside the cell should enable more sophisticated manipulation of cellular processes whenever real-time measurements of cellular variables are possible.
Medical subject headings
- Gene Regulatory Networks
- Models, Genetic
- Saccharomyces cerevisiae