Homing of placenta-derived mesenchymal stem cells after perinatal intracerebral transplantation in a rat model.

Abstract

The aim of this study is to assess early homing of placenta-derived stem cells after perinatal intracerebral transplantation in rats. Neonatal Wistar rats (2-4 days old) were anesthetized, and 250,000 human placenta-derived mesenchymal stem cells (MSC) injected into the lateral ventricle or the paraventricular white matter using a stereotactic frame. Donor MSC were detected by immunohistochemistry using an antihuman HLA-ABC antibody. In all, 84% of the animals survived the transplantation. Donor cells were detected in the brain ventricle 1-2 hours posttransplantation. After 4 hours, donor cells migrated throughout the ventricular system. At 1-4 weeks after transplantation, some cells had migrated into the periventricular white matter. Human placenta-derived MSC were successfully transplanted into the lateral ventricles of neonatal rats. Donor cells survived, homed, and migrated in the recipient brains. Proliferation and differentiation analysis and functional tests will assess the therapeutic effects of stem cell transplantation.