Cyanine fluorophore derivatives with enhanced photostability.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 22081126.
- Also identified by DOI 10.1038/nmeth.1774 and PMC identifier 3433158.
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Abstract
Fluorescence applications requiring high photostability often depend on the use of solution additives to enhance fluorophore performance. Here we demonstrate that the direct or proximal conjugation of cyclooctatetraene (COT), 4-nitrobenzyl alcohol (NBA) or Trolox to the cyanine fluorophore Cy5 dramatically enhanced fluorophore photostability without otherwise affecting its native spectral characteristics. Such conjugation is a powerful means of improving the robustness of fluorescence-based applications demanding long-lived, nonblinking fluorescence emission.
Medical subject headings
- Carbocyanines
- Fluorescent Dyes