Embryonic stem cell-based mapping of developmental transcriptional programs.
basic_science · Level V
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- Record sourced from PubMed, PMID 22081127.
- Also identified by DOI 10.1038/nmeth.1775 and PMC identifier 3228994.
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Abstract
The study of developmentally regulated transcription factors by chromatin immunoprecipitation and deep sequencing (ChIP-seq) faces two major obstacles: availability of ChIP-grade antibodies and access to sufficient number of cells. We describe versatile genome-wide analysis of transcription-factor binding sites by combining directed differentiation of embryonic stem cells and inducible expression of tagged proteins. We demonstrate its utility by mapping DNA-binding sites of transcription factors involved in motor neuron specification.
Medical subject headings
- Embryonic Stem Cells
- Gene Expression Regulation, Developmental
- Transcription Factors