Sensitive and specific single-molecule sequencing of 5-hydroxymethylcytosine.
basic_science · Level V
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- Record sourced from PubMed, PMID 22101853.
- Also identified by DOI 10.1038/nmeth.1779 and PMC identifier 3646335.
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Abstract
We describe strand-specific, base-resolution detection of 5-hydroxymethylcytosine (5-hmC) in genomic DNA with single-molecule sensitivity, combining a bioorthogonal, selective chemical labeling method of 5-hmC with single-molecule, real-time (SMRT) DNA sequencing. The chemical labeling not only allows affinity enrichment of 5-hmC-containing DNA fragments but also enhances the kinetic signal of 5-hmC during SMRT sequencing. We applied the approach to sequence 5-hmC in a genomic DNA sample with high confidence.
Medical subject headings
- Cytosine
- DNA
- Sequence Analysis, DNA